摘要
利用谷子基因组序列开发出SSR引物205对,其中171对可以稳定扩增出目的条带,149对有多态性。利用30个多态性SSR标记对41份谷子种质资源进行遗传多样性分析,共检测出291个等位变异,平均每个位点9.7个。谷子地方品种基因多样性指数平均为0.7907,育成品种基因多样性指数平均为0.7571。对41个谷子品种进行聚类,在遗传相似系数为0.164时聚为6组,与生态型基本一致。
In this study, 205 SSR primer pairs were designed from the genome sequence of foxtail mil- let. In which, 171 pairs could successfully amplify aimed fragment, and 149 pairs showed polymorphism. 41 foxtail millet germplasms were selected for genetic diversity analysis using 30 SSR markers. A total of 291 al- leles were detected out with an average of 9.7 per locus. The average genetic diversity index was 0. 7907 for landraces and 0. 7571 for divided into six groups at varieties. improved varieties. Cluster analysis showed that the 41 foxtail millet varieties were the similarity coefficient of 0. 164, which were consistent with the ecotypes of these
出处
《山东农业科学》
2013年第3期33-37,60,共6页
Shandong Agricultural Sciences
基金
山东省自然科学基金项目"谷子基因组SSR序列的分离
鉴定和引物开发"(Q2007D05)
关键词
谷子
SSR标记
遗传多样性
聚类分析
Foxtail millet
SSR markers
Genetic diversity
Cluster analysis
