腹腔高压对大鼠肝功能的独立影响

Independent effect of intra-abdominal hypertension on hepatic function of rats

目的研究腹腔内压力升高对肝功能的影响,为临床治疗提供相关依据。方法将45只SD大鼠随机分为3组,即正常对照组,腹腔内压(IAP)10mmHg模型组,腹腔内压(IAP)20mmHg模型组,每组15只。采用氮气气腹法制作SD大鼠腹内高压模型。每组动物分别于1、2、4h末处死,检测血中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)含量,同时取肝组织制作HE染色切片行组织病理学检查。结果在腹内高压状态下,AST和ALT的含量均随着IAP的增加和持续时间的增加而增高;肝组织病理切片显示,肝组织细胞受损。IAP 20mmHg 4h组处死的大鼠,可见肝细胞点状坏死,炎症细胞浸润。结论腹腔高压可致肝损伤,且与腹腔压力和持续时间密切相关。

Objective To provide evidences for clinical treatment and to investigate the effects of intra-abdominal hypertension （IAH） on hepatic function. Methods To establish animal models of IAH,forty-five male SpragueDawley rats were divided randomly into three groups of 15 each： control group, IAP-10 mmHg group and IAP-20 mmHg group. Intraperitoneal injection of gaseous nitrogen was chosen to be the method of the animal modeling. Each of these three groups then were randomly divided into three groups of 5 each ： duration of 1 h group, 2 h group and 4 h group. ALT and AST levels were measured at the end of the experiments. Additionally,tissue specimens of liver were taken for histopathologic examinations. Results Under the circumstance of IAH,AST and ALT levels were getting significantly higher when IAP and duration were increasing. The histopathological observation revealed parenchymal injury in liver. Occasional parenchymal spotty necrosis and inflammatory cells were observed in the IAP-20 mmHg 4 h group. Conclusion The hepatic function damage may be positively correlated with the levels of increased IAP and its duration of time.