Th1/Th2细胞因子在大鼠同种异体肾移植中的动态变化及其预测排斥反应与耐受的价值研究

An study of the value of predicting rejection or tolerance in renal transplantation by monitoring the kinetic levels of Th1/Th2 cytokine in rat renal allograft

目的监测血清Th1/Th2细胞因子水平及其移植肾组织中的mRNA表达在大鼠同种异体肾移植中的动态变化,研究其预测排斥反应与免疫耐受的价值。方法 ELISA法测定血清中细胞因子IL-2、IFN-γ、IL-4、IL-10的表达水平;实时定量RT-PCR(Real-time quantitative reverse transcription PCR)监测IL-2、IFN-γ、IL-4、IL-10 mRNA在移植肾组织中的表达。结果Th1细胞因子IL-2、INF-γ在大鼠同种异体肾移植中,无论在血清中的表达水平或在移植肾组织中的mRNA表达水平与同系对照组及CsA组比较均显著升高,而与此对应Th2细胞因子IL-4、IL-10显著降低。结论动态监测Th1/Th2细胞因子在外周血清中的浓度或其mRNA在移植肾组织中的表达水平可预测和判断急性排斥反应的发生,Th1细胞因子水平升高提示受体与移植肾之间存在高免疫反应状态,有发生急性排斥反应的可能;Th2细胞因子水平升高提示受体与移植肾之间存在低免疫反应或耐受状态。

Objective To monitor the kinetic levels of Th1/Th2 cytokine in serum and their mRNA expression in renal a11ograft, re- search the value of predicting rejection or tolerance in renal transplantation. Methods The cytokines of IL-2, IFN-γ, IL-4, IL-10 in ser- um was assayed by ELISA and their mRNA expression in renal allograft was monitored by real-time quantitative RT-PCR. Results The level of Thl cytokines （ IL-2, IFN-γ） in serum or their mRNA expression in renal allograft was significantly higher than that in the con- trol group. On the contrary,the level of Th2 cytokines（ IL-4, IL-10 ）was significantly reduced. Conclusion Monitoring the kinetic level of Th1/Th2 cytokine in serum and their mRNA expression in renal allograft could predict or diagnose rejection occurring. The rising of Thl cytokines level showed that hyperresponsive immune status existed in recipient against renal allograft and there was the possibility of acute rejection occurring. The rising of Th2 cytokines level showed that hyporesponsive immune status or tolerance existed in recipient against renal allograft.