^(99)Tc^m标记右旋糖苷衍生物DCM-1的淋巴结摄取和显像

Lymph Nodes Distribution and Imaging Study of ^(99)Tc^m Labeled Dextran Conjugate DCM-1

采用99 Tcm标记连接有S-半胱氨酸基团的甘露糖基化右旋糖苷衍生物(Dextran-S-Cysteine-Mannose,DCM),得到99 Tcm-(CO)3-DCM-1,在此基础上,进行了不同注射剂量的99 Tcm-(CO)3-DCM-1在正常小鼠体内分布和在Wistar大鼠淋巴结显像实验。实验结果表明,99 Tcm-(CO)3-DCM-1在正常小鼠的淋巴结摄取对注射剂量很敏感,当注射剂量(包括注射化学量和注射体积)减少时,前哨淋巴结(Sentinel Lymph Node,SLN)的摄取率提高,SLN提取率也相应提高。显像结果表明,减少标记物的注射剂量可以提高SLN显像效果。以上结果提示:99 Tcm-(CO)3-DCM-1的SLN摄取与提取率较高,SLN显像清晰,具有应用于SLN显像的潜在价值,值得进一步研究。

To evaluate the potential application of 99Tcm labeled mannosylated dextran conjugates with S-Cysteine（Dextran-S-Cysteine-Mannose,DCM）for sentinel lymph node（SLN）imaging,99Tcm-（CO）3-DCM-1 was prepared via [99Tcm（CO）3]＋ precursor synthesized by Isolink kit.Then,the effect of injected dosage on SLN uptake was studied.The result of biodistribution demonstrated that the biological behaviour of 99Tcm-（CO）3-DCM-1 was very sensitive to the injected dosage.When the injected dosage decreased,the uptake of SLN and the PE% increased correspondingly.The result of SLN SPECT imaging study was in accordance with that of biodistribution study.High SLN uptake and PE% of 99Tcm-（CO）3-DCM-1 showed its promising properties as SLN imaging agent and it was worth to have further investigation.