胆甾醇辛酸酯-辛酰基-1-^(13)C的合成

Synthesis of Cholesteryl Octanoate-octanoyl-1-^(13)C

采用缩合与催化的组合方式,利用胆固醇(Ch-OH)与辛酸-1-13 C反应,合成胆甾醇辛酸酯-辛酰基-1-13 C(ChO-13 C),产率84.1%,13 C丰度99.0%,光学纯度99.0%,化学纯度99.5%。用HPLC-ELSD、LC-MS和1 H NMR对产品进行表征,确定产品为胆甾醇辛酸酯结构。

Cholesteryl octanoate-octanoyl-1-13C was synthesized by a method of coupling-catalysis.The yield of 13C labeled Cholesteryl octanoate was 84.1%（based on Cholesterol）,with 99.0% atom 13C 99.0% optical purity and 99.5% chemical purity.The product was characterized by HPLC-ELSD,LC-MS and 1H NMR.The structure and 13C isotopic enrichment of it was determined and measured by LC-MS and 1H NMR.