酸性蛋白酶产生菌的筛选及其部分性质研究

Screening of acid protease strain and biochemical properties

以市售固体酒曲为实验材料,采用酪蛋白溶圈法及福林酚法对高产蛋白菌株进行了筛选,并对其部分生化性质进行了研究.结果表明:筛选得到的菌株DBM2初步鉴定为芽孢杆菌,其最适温度为30℃,最适pH值为5,为酸性蛋白酶产生菌.

Used solid koji as experimental materials, strains with high activity of acid protease were selected by lucency circle of casein plate, followed with Foline- phenol method, and researched some biochemical properties. The results showed that the strain DBM2 preliminary identified as Bacillus produce acid protease, the optimum temperature is 30 ℃, the optimum pH value is 5.