甲硫氨酸脑啡肽和抗δ阿片受体抗体对小鼠骨髓瘤细胞信号传递系统的作用

The Role of Methionine-enkephalin and Antibody Against Opioid Receptor on Signal Transduction System in Mouse NS-1 Cells

为了探讨阿片肽与细胞表面受体结合后所产生的生物效应及其机制 ,用不同浓度甲硫氨酸脑啡肽 ( MENK)及抗 δ阿片受体单克隆抗体处理小鼠的骨髓瘤细胞 ( NS- 1 ) ,然后测定蛋白激酶A( PKA) ,蛋白激酶 C( PKC)活性及三磷酸肌醇 ( IP3 )含量 .研究结果表明 ,NENK可升高细胞胞浆及胞膜 PKA的活性 ,且这一作用可被抗δ阿片受体抗体所拮抗 .MENK对 PKC影响呈双向反应 ,0 .1 μmol/L MENK可以升高胞浆 PKC活性 ,但却明显降低胞膜 PKC活性 ;在 MENK浓度为 1 0μmol/L时则情况刚好相反 .1 μmol/L的 MENK可明显降低胞浆及胞膜 PKC活性 ,抗体可拮抗这种下调作用 .MENK可降低细胞内 IP3 的含量 ,且这一作用可被抗δ阿片受体抗体所拮抗 .由此可以推论 :MENK在与 δ阿片受体结合后 ,可以经过多种信号传导系统来调节细胞功能 ,从而产生不同的生物效应 .

To investigate the biological effects and mechanisms brought about by the binding of opioid to cell surface receptors,the mouse NS\|1 cells were treated with different concentrations of methionine\|enkephalin (MENK) and monoclonal antibody against delta opioid receptor.The activity of protein kinase A(PKA) and protein kinase C(PKC),and the level of inositol trisphosphate(IP 3) were measured.The results showed that MENK could enhance the activity of PKA in both of cytoplasm and membrane,which was antagonized by the antibody.A diphase effect of MENK on PKC was noted.0 1 μmol/L of MENK could increase the activity of PKC in cytoplasm and lower the activity of PKC in membrane.However,an opposite effect of MENK on PKC could be observed at the concentration of 10 μmol/L.The activity of PKC in both of cytoplasm and membrane could obviously be down\|regulated by MENK at the concentration of 1 μmol/L and the antibody could reverse the effects.MENK could reduce the level of IP 3 in cells and the effect could be antagonized by the antibody.Coupled with all findings,it might be appraised that MENK could modulate the function of cells via various signal transduction systems and therefore result in different biological effects.