摘要
本文测定了彩绒革盖菌在PDY液体培养基中的愈创木酚氧化酶活性。在 30℃ ,1 1 0r/min ,恒温振荡培养条件下 ,第 1 6天达产酶高峰 ,酶活性 2 1 8.0u ;酶作用的最适pH为 4.0 ;最适温度 30℃ ;Ba2 +、Mg2 +、Cu2 +等离子对愈创木酚氧化酶有激活作用 。
The guaiccal oxidase activity of Corilus versicolor was studied when it was cultured in PDY fluid medium. The peak of the enzyme production appeared in the 16th days under the castant temperature of 30 ℃ and vibration(110 r/min), the maxmum enzyme activity was 218.0 u. The optimum temperature was 30 ℃ and the optimum pH was 4.0, Mn 2+ ,Ba 2+ ,Mg 2+ ,Cu 2+ were activators for guaiccal oxidase, whereas Ag + obviously inhibited the enzyme activity.
出处
《植物学通报》
CSCD
2000年第5期462-465,共4页
Chinese Bulletin of Botany
