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高氧对胎鼠远端肺上皮细胞液体转运功能及上皮钠通道表达的影响 被引量:1

The effect of hyperoxia on fluid transport by fetal distal lung epithelia
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摘要 目的探讨高氧对胎鼠远端肺上皮(FDLE)液体转运功能及上皮钠通道(ENaC)表达的影响。方法分离提取大鼠FDLE细胞,随机分成高氧组和空气组,分别在氧浓度为85%和21%的细胞培养箱中原代培养。检测高氧暴露24h和48h经FDLE细胞单层液体转运量的变化。应用Western blot方法检测高氧暴露后α-ENaC蛋白表达的变化。结果高氧48h使大鼠FDLE液体转运增加(1.78±0.19VS.1.06±0.11,P〈0.001),并且该促进作用可被阿米洛利抑制。高氧24hFDLE中α-ENaC蛋白表达与空气组比较差异无统计学意义(O.44±0.04VS.0.40±0.04,P=0.22),而高氧48hα-ENaC蛋白表达显著低于空气组(0.35±0.03VS.0.47±0.06,P=0.03)。结论高氧增强了FDLE液体转运功能,且以阿米洛利敏感性液体转运增加为主。该促进作用并非通过增加α-ENaC蛋白表达来实现。 Objective To investigate the effect of hyperoxia on-fluid transport by fetal distal lung epithelia(FDLE) and the expression of epithelial sodium channel(ENaC) in these cells. Methods FDLE were isolated and randomized into hyperoxia group and normoxia group, which were primarily cultured under hyperoxic or normoxic conditions, respectively. Fluid transport was measured using monolayers of FDLE cultured on Transwell permeable inserts. Western blot was applied to examine the α-ENaC expression. Results Fluid transport across monolayer of FDLE was increased in cells exposed to hyperoxia compaired with cells cultured in normoxic conditions( 1.78 ±0. 19 vs 1.06 ±0. 11 ,P 〈0. 001 ). Amiloride significantly decreased the fluid transport in both of the hyperoxia and normoxia groups, but in the presence of amiloride there were no difference between the two groups. The expression of α-ENaC was inhibited by hyperoxia to some extent( 24h :0. 44 ± 0.04 vs 0. 40 ± 0.04,P=0.22; 48h:0.35 ±0.03 vs 0.47 ±0.06,P =0.03). Conclusion Hyperoxia enhanced total and amiloride-sensitive fluid transport by FDLE. However,the expression of α-ENaC decreased in these cells.
出处 《国际儿科学杂志》 2013年第2期201-203,207,共4页 International Journal of Pediatrics
基金 国家自然科学基金资助项目(30872781)
关键词 高氧 胎鼠远端肺上皮细胞 液体转运 上皮钠通道 Hyperoxia Fetal distal lung epithelia Fluid transport Epithelial sodium channel
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参考文献19

  • 1Hahn WH, Chang JY, Chang YS, et al. Recent trends in neonatal mortality in very low birth weight Korean infants:in comparison with Japan and the USA. J Korean Med Sci,2011,26 (4) :467-473.
  • 2Walsh MC,Yao Q,Gettner P,et al. Impact of a physiologic defini- tion on bronchopulmonary dysplasia rates. Pediatrics,2004,114(5 ) : 1305-1311.
  • 3Adams EW, Harrison MC, Counsell S J, et al. Increased lung water and tissue damage in bronchopulmonary dysplasia. J Pediatr, 2004, 145 (4) :503-507.
  • 4Galliard EA, Shaw NJ, Wallace HL, et al. Electrical potential differ- ence across the nasal epithelium is reduced in premature infants with chronic lung disease but is not associated with lower airway inflam- marion. Pediatr Res,2007,61 ( 1 ) :77-82.
  • 5Thome UH, Bischoff A,Maier L,et al. Amiloride-sensitive nasal po- tential difference is not changed by estmdiol and progesterone re- placement but relates to BPD or death in a randomized trial on pre- term infants. Pediatr Res ,2006,60( 5 ) :619-623.
  • 6Hummler E, Barker P, Gatzy J, et al. Early death due to defective neonatal lung liquid clearance in alpha- ENaC-deficient mice. Nat Genet, 1996,12 (3) :325-328.
  • 7Thome UH, Davis IC, Nguyen SV, et al. Modulation of sodium trans- port in fetal alveolar epithelial cells by oxygen and corticosterone. Am J Physiol Lung Cell Mol Physiol,2003,284(2) :L376-385.
  • 8Elias N, Rafii B, Rahman M, et al. The role of a-, 13-, and r-ENaC subunits in distal lung epithelial fluid absorption induced by pulmo- nary edema fluid. Am J Physiol Lung Cell Mol Physiol, 2007,293 (3) :L537-545.
  • 9Gandhi SG, Rafii B, Harris MS, et al. Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung. Am J Physiol Lung Cell Mol Physio1,2007,293 ( 3 ) : L651-659.
  • 10Eaton DC, Helms MN, Koval M, et al. The contribution of epithelial sodium channels to alveolar function in health and disease. Annu Rev Physiol, 2009,71 : 403 -423.

二级参考文献20

  • 1Hahn WH,Chang JY, Chang YS, et al. Recent trends in neo- natal mortality in very low birth weight Korean infants:in corn-parison with Japan and the USA. J Korean Med Sci,2011,26 (4) :467-473.
  • 2Walsh MC, Yao Q,Gettner P,et al. Impact of a physiologic def- inition on bronchopulmonary dysplasia rates. Pediatrics,2004, 114(5) :1305-1311.
  • 3Canessa CM, Schild L, Buell G, et al. Amiloride-sensitive epi- thelial Na + channel is made of three homologous subunits. Na- ture, 1994,367 (6462) :463-67.
  • 4Hummler E,Barker P, Gatzy J, et al. Early death due to defec- tive neonatal lung liquid clearance in alpha-ENaC-deficient mice. Nat Genet, 1996,12 ( 3 ) : 325-328.
  • 5Matthay MA, Folkesson HG, Clerici C. Lung epithelial fluid transport and the resolution of pulmonary edema. Physiol Rev, 2002,82( 3 ) :569-600.
  • 6Galliard EA, Shaw NJ, Wallace HL, et al. Electrical potential difference across the nasal epithelium is reduced in premature infants with chronic lung disease but is not associated with lower airway inflammation. Pediatr Res,2007,61 ( 1 ) :77-82.
  • 7Thome UH, Bischoff A, Maier L, et al. Amiloride-sensitive na- sal potential difference is not changed by estradiol and proges- terone replacement but relates to BPD or death in a randomized trial on preterm infants. Pediatr Res,2006,60 ( 5 ) :619 4523.
  • 8Yue G,Russell WJ, Benos DJ, et al. Increased expression and activity of Na channels in alveolar type II cells of hyperoxic rats. Proc Natl Acad Sci USA, 1995,92( 18 ) :8418-8422.
  • 9Li TB, Koshy S, Folkesson HG. Involvement of cENaC and Nedd4-2 in the conversion from lung fluid secretion to fluid ab- sorption at birth in the rat as assayed by RNA interference anal- ysis. Am J Physiol Lung Cell Mol Physiol, 2007,293 (4): L1069-1078.
  • 10Nie HG,Tucker T, Su XF, et al. Expression and regulation of epithelial Na + channels by nucleotides in pleural mesothelialcells. Am J Respir Cell Mol Biol,2009,40(5 ) :543-554.

同被引文献16

  • 1Natarajan G, Pappas A, Shankaran S, et al. Outcomes of extremely low birth weight infants with bronchopulmonary dysplasia: impact of the physiologic definition [ J ]. Early Hum Dev ,2012,88 ( 7 ) :509 - 515.
  • 2Willis BC, Liebler JM, Luby-Phelps K, et al. Induction of epithelial-me- senchymal transition in alveolar epithelial ceils by transforming growth factor-betal :potential role in idiopathic pulmonary fibrosis [ J 1. Am J Patho1,2005,166 ( 5 ) : 1321 - 1332.
  • 3I-Iansmann G,Fernandez-Gonzalez A, Aslam M, et al. Mesenchymal stem cell-mediated reversal of bronchopulmonary dysplasia and associated pulmonary hypertension [ J ]. Pulm Circ,2012,2 ( 2 ) : 170 - 181.
  • 4Xu W,Yang N, Pan L, et al. The expression of HoxB5 and its role in neonatal rats with chronic lung disease [ J]. Fetal Pediatr Pathol,2012, 31(1):11 -20.
  • 5Northway WH Jr, Rosan RC, Porter DY. Pulmonary disease following res- pirator therapy of hyaline-membrane disease. Bronchopulmonary dyspla- sia[ J]. N Engl J Med,1967,276(7) :357 -368.
  • 6Hadchouel A, Franco-Montoya ML, Delacourt C. Altered lung develop- ment in bronchopulmonary dysplasia[ J]. Birth Defects Res A Clin Mol Teratol,2014,100(3 ) : 158 - 167.
  • 7Zhu Y,Fu J, You K, et al. Changes in pulmonary tissue structure and KL-6/MUC1 expression in a newborn rat model of hyperoxia-induced bronchopulmonary dysplasia [ J ]. Exp Lung Res, 2013,39 ( 10 ) : 417 - 426.
  • 8Joss-Moore L, Carroll T, Yang , et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups [ J ]. Pediatr Res, 2013,73 (5) :612 -620.
  • 9Ahola T, Lapatto R, Raivio KO, et al. N-acetylcysteine does not prevent bronchopulmonary dysplasia in immature infants: a randomized con- trolled trim [ J 1. J Pediatr,2003,143 (6) :713 - 719.
  • 10Fehrenbach H. Alveolar epithelial type II cell : defender of the alveolus revisited[J]. Respir Res,2001,2(1 ) :33 -46.

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