人血管内皮抑素基因的克隆及其在原核中的表达

Cloning and expression of endostatin in escherichia coli

以人胎肝组织为材料 ,提取总RNA ,经反转录和PCR扩增 ,得到血管内皮抑素基因 ,序列分析表明 ,与国外文献报道一致。将其克隆到大肠杆菌表达系统pGEX -KG ,分别在DH5α、BL - 2 1菌中以GST

The total RNA was extracted from the fetal liver and the endostatin gene was amplifieated by RT-PCR.After identified by DNA sequencing,it was cloned into the expression vector pGEX-KG and then transformed into the host cells E.coli DH5α and BL21.Subsequently the recombinant GST-fusion protein was expressed and purified.