从栀子细胞培养物中分离多糖最佳工艺条件的研究

Studies on the optimum technology for isolation of polysaccharides from cultured cells ofGardenia jasminoides eills

对栀子细胞培养物多糖的提取、纯化条件进行优化研究 ,结果表明 ,提取的最佳工艺是 80℃下的碱性水中浸提 2h ,加水量为 50倍 ,醇析用乙醇的浓度为 70 % - 80 % ,蛋白质去除过程中氯仿 /正丁醇 (V/V) ,样品 /氯仿 -正丁醇 (V/V)分别为 1∶0 4和 1∶0 2 4时 。

The optimum technology of extraction and purification of polysaccharides from Gardenia jasminoides eills was studied.The results show that the optimum technology of isolation is to extract about 2 hours in 50 times water at 80℃,to separate polysaccharides out with 70%-80% ethanol,and to remove proteins when chloroform/n-butanol is 1:0.24 and the sample/chloroform-n-butanol is 1:0.4.