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荞麦愈伤组织分化与过氧化物酶活性及其同工酶关系的研究(英文) 被引量:2

STUDY ON PEROXIDASE ACTIVITY AND ITS ISOENZYMES IN CALLUS DIFFERENTIATION OF COMMON BUCKWHEAT
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摘要 在附加不同外源激素的培养基中 ,龙山荞的子叶愈伤组织表现出不同的分化状态 :( 1 )不分化 ;( 2 )分化根 ;( 3)分化芽 ;( 4 )分化全苗。四种愈伤组织的过氧化物酶活性的相对比值为 1 :1 .47:5 .95 :7.78,并且过氧化物酶同工酶谱也存在明显差异。酶带 4’为分化愈伤组织所特有 ;酶带 2’为未分化和分化愈伤组织所共有 ;在分化根和未分化愈伤组织中缺少酶带 1’ ;未分化愈伤组织比分化愈伤组织多出酶带 3’。 On various mediums with different variety and level of phytohormone, cotyledon callus of Longshan common buckwheat showed different differentiation states: (1) no differentiation took place; (2) root differentiation; (3) bud differentiation and (4) plantlet differentiation. The ratio of peroxidase activity in 4 kinds of calli is 1:1.47:5.95:7.78. There appeared also significant difference among their zymogram of peroxidase isoenzyme. Band 4' in zymogram is characteristic for the differentiated callus; band 2' is common for the undifferentiated and the differentiated; band 1' is of shortage for the differentiated root and the undifferentiated; band 3' is an extra for the undifferentiated.
出处 《木本植物研究》 CSCD 北大核心 2000年第4期416-419,共4页
基金 TheprojectisfinancedbyShanxiNaturalScienceFunds( 96 10 2 0 )
关键词 荞麦 组织培养 过氧化物酶 同工酶 愈伤组织分化 Common buckwheat Tissue culture Peroxidase Peroxidase isoenzyme
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