咖啡因加热休克诱导皱纹盘鲍多倍体的研究

Induction of Polyploid in the Pacific Abalone by Caffeine-Heat Shock Treatments

报道了用咖啡因加热休克抑制受精卵的第一极体的释放诱导皱纹盘鲍（Ｈａｌｉｏｔｉｓｄｉｓｃｕｓ ｈａｎｎａｉ Ｉｎｏ）多倍体的研究结果。皱纹盘鲍的卵在 ２１℃海水中受精，受精后 １０分钟开始处理，药物浓度分别为 ２．5ｍｍｏｌ／ Ｌ、５ｍｍｏｌ／ Ｌ、 １０ｍｍｏｌ／ Ｌ，热休克温度分别为 ２４℃、２６℃、２８℃，处理的持续时间为 １０分钟至 ３０分钟，共分 ５个时间段。结果表明，药物浓度５ｍｍｏｌ／ Ｌ和 １０ｍｍｏｌ／ Ｌ，处理温度２６℃，处理持续时间 １０～１５分钟，其三倍体诱导率可达６６．４％～７７．８％，综合评价指数为０．５７～０６１。在５ｍｍｏｌ／ Ｌ，２６℃条件下，处理持续时间 ３０分钟可以产生２．８％～３．６％的四倍体胚胎。

Polyploid of Pacific abalone, Haliotis discus hannai Ino, was induced by blocking Polar body I with caffeine-heat shock treatments. Fertilized eggs were treated with caffeine concentrations at: 2.5 mmol /L, 5 mmol /L and 10 mmol /L, heat shock temperatures at 24℃,26℃, 28℃, lasting for 10 min to 30 min, total 5 periods of time, beginning at 10 min post-fertilization. Both fertilizing and incubating at 21℃ sea water. The results of studies showed that the effective procedure for inducing triploid appeared to be 5 mmol /L and 10 mmol/L Caffeine concentration, heat shock temperature 26℃, duration 10 min to 15 min, all beginning at 10 min after insemination. The percentage of triploid was 64.6%-77.8%, Comprehensive efficiency index of polyploid induction was 0.57-0.61. Under the condition of 5 mmol /L caffeine concentration and the temperature 26℃ for 30 min duration at 10 min post-fertilization, 2.8%-3.6% tetraploid embryos could be produced.