白藜芦醇抗肿瘤作用的体外实验研究

Studies of Anti-Cancer Effect of Resveratrol in vitro

选用呼吸、消化、循环系统来源的 4种癌细胞株 :人肺癌PG细胞、人大肠上皮癌lovo细胞、人肝癌细胞HepG2、人白血病HL 60细胞以及小鼠成纤维细胞 3T3作为研究对象 ,检测Res的体外抗癌活性 .采用MTT法检测细胞的生长增殖活性 .结果显示 ,Res不影响正常细胞 ( 3T3)的生长增殖活性、对PG、lovo细胞的生长增殖也不发生作用 ;但明显抑制HepG2、HL - 60细胞的生长增殖 .表明Res对癌细胞的作用因细胞的类型不同而异 ,并且只对部分癌细胞生长有抑制作用 ,即Res的抗癌作用具有癌种类的选择性 .此外 ,Res发挥作用的时间。

To study the anti cancer effect of resveratrol in vitro ,five kinds of tumor cells(lovo,PG,HL 60, HepG2 and 3T3) were chosen and MTT assay was used to detect the influence of resveratrol on the growth and proliferation of the tumor cells.The results show that resveratrol significantly inhibits the proliferation of HL 60 and HepG2 cells,but has no influence on normal 3T3 cells and PG cells,indicating that the anti cancer effect of resveratrol has seclectivity.On the other hand, the effective period and concentration of resveratrol are different for different kinds of cells.