海洋细菌11211的生长条件及其几丁质酶研究

Studies on the Growth and Chitinase Production of a Marine Baterium 11211

从南海海水中分离到产几丁质酶的弧菌 (Vibriosp .) ,研究了菌株 112 11的生长条件和产酶条件 . 112 11菌生长需要Na+,海水的其他成分对菌生长也有影响 .胶体几丁质可诱导几丁质酶产生 .在几丁质培养基中 112 11菌的产酶高峰为生长的第 7天 .经硫酸铵沉淀 ,SephadexG 10 0、DEAE 纤维素及几丁质柱层析 ,酶被纯化了 18倍 .经SDS PAGE分析 ,酶的相对分子质量约为 30 0 0 0 .该酶的反应动力学特征也被初步研究 .

Bacterium 11211 which produces chitinase was isolated from the South China Sea.The growth condition and chitinase production of bacteria were investigated.Na + was necessary for bacterium 11211 growth.Colloid chiyin could induce bacteria to produce chitinase.The growth of bacteria was slow in the medium containing colloid chitin as main carbon source,and the enzyme activity reached maximum on 7 d of incubation.Following the purification,the enzyme was characterized and its molecular weight was estimated by SDS PAGE.