摘要
从海洋细菌中筛选到一株产3-脱氧葡糖醛酮代谢酶活力较高的菌株Fs-4—C-3,并对其进行了最适产酶条件的研究。实验证明:以鱼肉蛋白胨为氮源、蔗糖为碳源,在培养基起始pH值为7.8~8.0,温度为28℃,盐度为5%的情况下,培养96h,产酶最高。3-DG可以诱导产酶。
A bacterial strain having 3-deoxyglucosone-metabolizing enzyme was selected from 31 marine bacterial stains. The conditions for enzyme production of the strain was examined. The optimal tempreture, initial pH. and cultivate time for enzyme formation were 28℃, pH7.8~8.0, and 96 hours respectively. Composition of the suitable medium was as following (%): Fish peptone 1.0, Sucrose 0.3, Yeast extract 0.2, NaCI 5.0.3-deoxyglucosone can induce formation of enzyme.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期321-324,共4页
Microbiology China
基金
国家自然科学基金资助项目!(No.29466012)