摘要
紫苏叶外植体在添加ＮＡＡ和 ２，４－Ｄ的 ＭＳ培养基上诱导分化愈伤组织，愈伤组织中迷迭香酸的含量为 ０．８５％，愈伤组织干燥后经乙醇提取，乙酸乙酯萃取后，经Ｓｅｐｈａｄｅｘ ＬＨ－２０柱层析，最后获得了纯度为９５％的迷迭香酸。抑菌实验表明此法获得的迷迭香酸对大肠杆菌、金黄色葡萄球菌及立枯丝合菌的生长均有明显的抑制作用，其最低抑制浓度分别为３００、４００、及８００μｇ／ｍＬ。
Perilla frutescens callus were induced from leave explants on MS medium suplemented with NAA and 2,4-D. The Rosmarinic acid (RosA) content of dried callus was 0.85%. The RosA was extracted from the callus with 80% alcohol and purified through extraction with ethyl acetate and a Sephadex LH-20 column chromatography. The parity of the final product was 95% as analyzed by HPLC RosA could inhibit the growths of Escherichia colt, Staphylococcus aueris and Rhizotonia with MICs of 300, 400, 800μg/mL, respectively.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期324-327,共4页
Microbiology China
基金
山东省自然科学基金
青岛大学校内基金资助项目
关键词
紫苏
愈伤组织
迷迭香酸
抗菌活性
Perilla frutescens
Callus
Rosmarinic acid
Antifugal and antibacterial activities
