平菇汤培养基的研究及应用

STUDY AND APPLICATION ON THE MUSHROOM BROTH CULTURE

研究以平菇汤为基础成分，代替动物组织提取物，制备平菇液体培养基，平菇血琼脂培养基。平菇血琼脂培养基对１４０份痰及中段尿标本阳性菌的检出率为３４．３％，传统血琼脂培养基对阳性菌的检出率为３２．１％。平菇液体培养基和普通营养肉汤培养基同时对８０份分泌物标本培养，其检出率分别为３８．８％和３６．３％（ｐ＞０．０５）。平菇汤培养基制备简便，成本低廉，有较好的实用价值。

Mushroom broth is used to replace animal tissue extract to produce mushroom culture solution and mushroom blood agar medium. For 140 case of sputum and urine culture, the positive rate on mushroom blood agar medium is 34.3%, while it on traditional blood agar medium is 32.1%. 80 case of secreta were cultured on mushroom culture solution and general broth culture,the positive rate being respectively 38.8% and 36.3% (p> 0.05). It shows that mushroom broth culture, which is processed conveniently and cheaply, has good practical value.