真核基因转录与转录调节相关复合物

Transcription of Eukaryotic Genes and Complexes Related to Transcription Regulation.

真核细胞核中转录因子与染色质模板如何相互作用调节基因转录是基因表达调控研究的一个中心问题 .近来的研究表明 ,参与基因转录的各种调节因子在核内形成多种复合物 ,如RNA聚合酶Ⅱ全酶、染色质重塑复合物、核小体以及增强小体等 .这些复合物之间相互作用 ,调节染色质结构 ,在染色质模板上进一步组装成转录复合物 ,参与转录调节的各个环节 ,调节转录复合物活性 .这些复合物的形成 ,整合了转录调节的各种信息 ,提高了转录调节效率 ,是真核基因有效、严格、有序表达的基础 .另一方面 ,这些复合物的存在给基因表达调控的研究提出了新问题 。

How transcription factors regulate gene transcription on chromatin is the central question of the study of gene expression regulation. Recent investigations showed that regulatory factors form different complex in nucleus such as RNA polymerase Ⅱ holoenzyme, chromatin remodeling complexes, nucleosome and enhanceosome, which participate each step of gene transcription and assemble into active transcription complex. Formation of these complexes integrates lots of information of transcription regulation and increase the efficiency of transcription, which is the basis of orderly efficient expression of genes. On the other hand, it is a challenge to study these transcription complexes and development of new technology is important.