蛋白凝胶基质的制备与基质内的血管生成反应

Preparation of Protein Gel Matrix and Angiogenesis of Vascular Endothelial Cell in the Gel.

利用肝素亲和层析从血清中提取玻璃粘连蛋白 (vitronectin) ,以硫酸铵沉淀法从血浆中粗提含纤维蛋白原的复合蛋白质组分 ,向血浆蛋白、胎牛血清和DMEM组成的复合成分中加入凝血酶 ,制成蛋白质凝胶 .观察血管内皮细胞在此基质上或在基质中的生长及在碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)的诱导下形成的血管样结构 .结果表明血管内皮细胞可粘附在此凝胶基质表面正常生长 ,在bFGF的诱导下 ,内皮细胞向胶内迁移、生长并形成管状结构 ,多个管状结构连接。

Vitronectin was purified from human plasma by heparin affinity chromatography and rough plasma protein containing fibrinogen was isolated from human plasma by (NH 4) 2SO 4 precipitation. Protein gel was generated by addition of thrombin to the complex composed of plasma protein, bovine fetal serum and DMEM.Bovine aortic endothelial cell(BAEC) was plated on the gel matrix and the growth,vascular formation of endothelial cell in the gel were observed. The results demonstrated that BAEC can adhere to the gel surface and grow normally as grow on the culture plate, while under the induction of bFGF, BAEC can migrate into the gel and form vascular like structure in the gel matrix. Many vascular like structures can link to each other to form capilary net structure.