大剂量地塞米松快速高效诱导巨噬细胞凋亡

Study on the Apoptosis of Macrophages Induced by Dexamethasone.

运用透射电镜、DNA琼脂糖凝胶电泳、流式细胞术、原位末端标记法 (TUNEL)染色等技术 ,检测了大剂量地塞米松处理小鼠腹腔巨噬细胞的各种变化 .结果显示 ,大剂量地塞米松处理的巨噬细胞发生胞体皱缩、染色质凝聚、胞质浓缩 ;TUNEL染色呈阳性 ;0 5h后DNA凝胶电泳即呈梯状条带 ;流式细胞术作周期分析出现凋亡峰等明显的凋亡特征 .并且随处理时间延长凋亡率升高 .结果表明 ,大剂量地塞米松快速。

By transmission electron microscopy, DNA agarose gel electrophoresis, flow cytometry and TUNEL staining, changes of the murine peritoneal macrophages treated with high dose of dexamethasone were observed. The results clearly showed that, treated with high dose of dexamethasone, the macrophages presented various changes in ultrastructure: chromatin condensed and abutted sharply against the nuclear membrane, and cytoplasm condensed. Half hour after dexamethasone treatment, DNA ladder was visualized by agarose gel electrophoresis. The macrophages displayed TUNEL positive. The characteristic apoptosis peak of DNA was showed by flow cytometry. The results indicated that the macrophages treated with high dose of dexamesathone may develop apoptosis rapidly and efficiently.