一种简便的考马斯亮蓝G250蛋白质染色方法

A Simple Method of Protein Staining with CBB G250 .

介绍一种快速、简便、几乎无背景的考马斯亮蓝G2 5 0 (CBBG2 5 0 )染色方法 .该方法所用试剂仅为稀盐酸和CBBG2 5 0 ,CBBG2 5 0的工作浓度为 0 0 0 15 % ,灵敏度达 0 0 2 μg/带 ,染色 2h达 70 % ,4h以上或染色过夜即可充分染色 .与以往的考马斯亮蓝染色方法相比 ,该方法有经济方便、灵敏度高、几乎无背景等优点 。

A simple method of protein staining with Coomassie Brilliant Blue G250 is introduced, which had almost no background,needed less time and only two reagents. The working concentration of Coomassie Brilliant Blue G250 in the staining system with diluted hydrochloric acid was 0 0015%, and the sensitivity was 0 02 μg protein per electrophoresis zone.