APP17肽对AT-8在糖尿病小鼠脑内分布的影响

THE EFFECT OF App17 PEPTIDE ON AT-8 DISTRIBUTION IN BRAIN TISSUES OF DIABETIC MICE

为了观察 APP17肽对糖尿病小鼠 Tau蛋白 Ser2 0 2 / Thr2 0 5磷酸化的影响 ,用链脲佐菌素 (选择性地破坏胰岛β-细胞 ,导致糖尿病 )诱发小鼠糖尿病模型 ,并皮下注射 APP17肽 ( β-淀粉样肽前体蛋白 3 19～ 3 3 5 )给予治疗。 4周后取脑组织进行 AT-8(抗 Ser2 0 2 / Thr2 0 5特异单抗 )免疫组化反应。结果证明 :糖尿病组 AT-8阳性反应细胞广泛分布于压后颗粒皮层、海马、丘脑、下丘脑等部位 ,而正常对照组及 APP17肽治疗组仅在压后颗粒皮层、海马可见阳性反应细胞 ,且着色淡。本研究提示 ,糖尿病脑内多个区域的神经元存在较多的 AT-8阳性细胞 ,而 APP17肽可使 AT-8阳性反应细胞出现的部位和数量正常化。

The purpose of the present work is to observe whether Tau protein Ser202/Thr205 is hyperphosphorylated in brain tissues of diabetic mice and to study the effect of App17 peptide. Mouse diabetic model was produced with streptozotocin, and App17 peptide as a treatment was injected subcutaneously into diabetic mice. Four weeks later, fixative was injected intravascularly into the mice, the brain was removed and crystat sections prepared. Immunohistochemical staining was done with AT 8. In the brains of diabetic mice positive AT 8 reacting neurons were numerous, darkly stained, and widely distributed in retrosplenial granular cortex, hippocampus, thalamus et al., while in normal mice and App17 peptide treated diabetic mice positive cells were scarce and poorly stained. Tau protein is hyperphosphorylated at Ser202/Thr205 site and widely distributed in the brains of diabetic mice, while App17 peptide can normalize the expression of AT 8 positive cells.