类球红细菌产辅酶Q_(10)发酵工艺的优化

Optimization of fermentation process for Rhodobacter sphaeroides producing Coenzyme Q_(10)

类球红细菌(Rhodobacter sphaeroides)是一类高产辅酶Q10的光合细菌,该研究对R.sphaeroides EIM-8产辅酶Q10的发酵条件和培养基进行了优化。采用单因素结合响应面设计优化后的培养基组成为:葡萄糖27.8 g/L、(NH4)2SO4 4.9 g/L、谷氨酸4.7 g/L、玉米浆粉2.5 g/L、MgSO49.5 g/L、FeSO41.5 g/L,NaCl 3.5 g/L,KH2PO44.0 g/L、辅液15.0 mL/L;通过单因素试验方法确定的培养条件为:pH 6.5、温度32℃、接种量10%、装液量40mL/250 mL,此条件下辅酶Q10产量可达128.9 mg/L,比优化前提高了89.0%。

Rhodobacter sphaeroides is a kind of high yield coenzyme Q10 of photosynthetic bacteria. The medium composition and fermentation conditions for production of coenzyme Q10 by R. sphaeroides EIM-8 were optimized. Op- timization of medium was performed by single factor and response surface design. The optimized medium composition was as follows： glucose 27. 8 g/L, （NH4）2SO4 4.9 g/L, glutamic acid 4.7 g/L, corn steep liquor 2.5 g/L, MgSO4 9.5 g/L, FeSO4 1.5 g/L, NaC1 3.5 g/L, KH2PO4 4.0 g/L, auxiliary liquid 15.0 mL/L. The culture condition de- termined by single factor experiment was as follows ： initial pH 6. 5, temperature 32 ℃, inoculum 10% , medium vol- ume 40 mL/250 mL. Using the described medium and condition, eoenzyme Ql0 yield could reach 128.9 mg/L which was 89.0% higher than results prior to optimization.