吸烟状态对AMI患者发病6h内炎症因子表达的影响

Effect of cigarette smoking on the expression of inflammatory cytokines in acute myocardial infarction within 6 hours

目的探讨吸烟状态对急性心肌梗死（AMI）患者发病6h内血清IL-1、hs—CRP、TNF-α和IL-10表达水平的影响。方法采用双抗体夹心ABC—ELISA法测定IL-1、IL-10，放射免疫分析测定TNF-α，速率散热比浊法测定hs-CRP，3组均数比较采用单因素方差分析，采用q检验对组间进行两两比较，计数资料采用r检验。结果方差分析结果显示，3组比较差异有统计学意义（P〈0．01），q检验进一步显示，3组IL-1、hs-CRP、TNF-α和IL-10两两之间的比较差异均有统计学意义（P〈0．05）。不吸烟AMI组患者血清中IL-1、hs-CRP、TNF-α和IL-10表达水平高于正常对照组（P〈0．05），吸烟AMI组患者血清中IL-1、hs—CRP、TNF-α和IL-10表达水平高于不吸烟组和正常对照组。结论吸烟可上调AMI患者血清IL-1、hs—CRP、TNF-α和IL-10表达水平。

Objective To explore the effect of cigarette smoking on the expression of serum interleukin - 1 （ IL - 1 ） , high - sensitivity C - reactive protein （ hs - CRP）, tumor necrosis factor - α （TNF - α） and IL - 10levels in acute myocardial infarction within 6 hours. Methods Double - antibody sandwich ABC - ELISA was used to detect the expression levels of IL - 1 and IL - 10, the serum TNF -α and hs - CRP levels were measured by radioimmunoassay and rate nephelometry respectively. Variance analysis was used to compare the mean of three groups, the q test was used to compare the differences between groups. The enumeration data were analyzed by the chi - square test. Results Variance analysis showed statistical significances among the three groups （P 〈 0.01 ）. The q test showed statistical differences in the serum levels of IL - 1, hs - CRP, TNF - α and IL - 10 with multiple comparisons among the three groups. The serum levels of IL - 1, hs - CRP, TNF - α and IL - 10 were higher in no smoking AMI patients than in normal group （ P 〈 0.05 ）, the serum levels of IL - 1, hs - CRP, TNF -α and IL - 10 were significantly higher in smoking AMI patients than in no smoking AMI patients and normal group（ P 〈0.05 ）. Conclusion Smoking can increase the expression levels of serum IL - 1, hs - CRP, TNF - α and IL - 10.