Miconazole对破骨细胞在羟基磷灰石涂层吸收的影响

The effect of miconazole on the resorption ability of osteoclast on hydroxyapatite coating

目的:研究钙离子通道抑制剂miconazole对破骨细胞对羟基磷灰石涂层的吸收的影响。方法:在羟基磷灰石涂层表面诱导破骨细胞,破骨细胞形成后,分别加入不同浓度的miconazole细胞培养基,培养48h后,扫描电镜观察羟基磷灰石表层的吸收陷窝。结果:miconazole可抑制破骨细胞的羟基磷灰石涂层的吸收陷窝面积,且吸收陷窝面积随着miconazole浓度的增加而增加。结论:miconazole可抑制破骨细胞对羟基磷灰石涂层的吸收。

Objective： To research the effect of miconazole on the osteoclastic resorption ability on hydroxyap- atite （HA） coating surface. Methods： Osteoclasts were generated on HA coating surface. After osteo- clasts were generated, different concentrations of miconazole was added and cocuhured for 48h. The re-sorptlon pits on the surface was measured by SEM and the resorption area were measured. Results： Mi- conazole inhibited resorption area on HA coating surface in a dose dependent manner. Conclusion： Mi- conazole inhibited osteoclastic resorpiton on HA coating surface.