摘要
目的使用联合成像系统在亚细胞水平研究心肌细胞微区力学性质及细胞内钙离子的快速变化，并探讨两者之间的关系。方法应用原子力显微镜（AFM）和激光扫描共聚焦显微镜（LSCM）组合成的联合成像系统对急性分离的大鼠单个心室肌细胞微区力进行检测，同时测定细胞内钙离子浓度的变化，分析细胞微区力和细胞内钙离子浓度变化两者之间的相关性。结果观察到心肌细胞内钙离子浓度的快速变化引起了钙波的传递。通过AFM微悬臂的偏转可以计算出细胞微区力，细胞微区力的大小与细胞内钙离子浓度呈正相关（r=0．701，P=O．003）。结论联合成像系统可以同步研究心肌细胞微区力学及细胞内分子快速变化，进而在亚细胞水平研究心脏疾病的发病机制。
Objective To investigate the micro-mechanical properties of myocardial cell and rapid changes in intracellular calcium cation at the subcellular level with combination imaging system, and to explore the correlation between them above. Methods The micro-mechanics of acutely isolated single myocardial cells in rats were detected using the combination imaging system which was made up of atomic force microscope （AFM） and laser scanning confocal microscope （LSCM）, and the changes in intracellular calcium cation concentration were detected as well. The correlation between micro-mechanics of myocardial cell and changes in intraeellular calcium cation was then analyzed. Results The rapid changes in intracellular calcium cation concentration were found to cause the transmission of calcium waves. The strength of cell micro-region could be calculated by deflection of cantilever in AFM. And the strength of cell micro-region was positively correlated with intracellular calcium cation concentration （r=0.701, P=0.003）. Conclusion The combination imaging system can simultaneously investigate the micro-mechanics of myocardial cell and rapid changes in intracellular molecule, and provide the technique for further research on the pathogenesis of heart disease at the subcellular level.
出处
《中华生物医学工程杂志》
CAS
2012年第6期451-455,共5页
Chinese Journal of Biomedical Engineering
