人脐血和外周血单个核细胞增生活性及紫外线B照射抑制作用的研究

STUDY OF THE PROLIFERATIVE ACTIVITY AND THE INHIBITION EFFECT OF UVB IRRADIATION ON HUMAN CORD BLOOD AND PERIPHERAL BLOOD MONONUCLEAR CELLS

目的 :比较人脐血 (CB)和外周血 (PB)单个核细胞 (MCs)的增生活性及其紫外线 B照射 (UVB)在增生活性上的抑制作用。方法 :应用 PHA诱导细胞增生以 3 H- Td R结合检测增生活性 (cpm) ;应用 CBMCs和PBMCs的单向混合淋巴细胞培养 (ML C)测试各自的增生反应 (cpm) ,UVB照射后的 PHA刺激和 ML C抑制作用 ,以未照射细胞的活性百分数表示。结果 :人 CBMCs和 PBMCs cpm平均值无统计学差异 ,但单个实验的标准偏差均显示人 CBMCs的 cpm高于 PBMCs,二者对小剂量 U VB照射均十分敏感 ,CBMCs经 1.0 m J/ cm2～ 2 .0 m J/cm2 U VB照射后 ,PHA刺激和单向 ML C分别下降 5 0 %以下及 2 5 .6 %以下。结论 :CBMCs和 PBMCs不仅有类似反应 ,有可能 CBMCs比 PBMCs有更强的增生反应 ,因此 CB移植需预防 GVHD,U VB照射可明显灭活 CBMCs和PBMCs的增生反应 ,U VB照射可能是更为简便、敏感、安全、有效的体外预防 GVHD的方法 ,可能更适合 CB移植。

Objective:To compare the proliferative activity between human cord blood mononnclear cell(CBMCs) and peripheral blood mononuclear cell(PBMCs),and to study UVB inhibition effect on proliferativc activity.Methods:Using PHA stimulation, 3 H TdR incorporativc(cpm) presented proliferative activity.Using mixed lymphocyte culture(MLR) between CBMCs and PBMCs determine the proliferative activity (cpm).The inhibition effect of the UVB irradiation of CBMCs and PBMCs exprssed as % activity compared to controlled.Results:Results show PHA stimulation and MLC between CBMCs and PBMCs are not significantly different on means.But when the means and standard deviations of each experiment are analyzed individually,CBMCs response is significantly (P<0.01) increased in most of cases (>86%).Conclusion:The proliferative activity of CBMCs and PBMCs to PHA as well as in MLCs posses similar responses.But CBMCs responses to adults r irradiated PBMCs is greater than PBMCs.Therefore,GVHD prophylaxis may be critical in CB transplant.UVB irradiation (UVBR) can inactivity proliferative activity.UVBR might be a simple,sensitive,safe and efficient tool GVHD prophylaxis,especially suitable in CB transplants.