高效液相色谱法测定补肾暖阳颗粒中淫羊藿苷的含量

Determination of Icariin contents in Bushen Nuanyang granules by HPLC

目的:测定补肾暖阳颗粒中淫羊藿苷的含量。方法:采用高效液相色谱法(HPLC法)。色谱柱为C18柱(200mm×4.6mm,5μm),以乙腈-水(30:70)为流动相,检测波长为270nm。结果:淫羊藿苷在0.24～3.60μg范围内线性关系良好,加样回收率为98.30%,RSD为0.64%。结论:HPLC法简便、准确,可用于该制剂的质量控制。

Objective：To determine the contents of Icariin in Bushen Nuanyang granules.Methods：HPLC method was adopted and a C18 column（200mm×4.6mm,5μm）was used.The mobile phase was acetonitrile-water（30：70）.The detection wavelength was at 270 nm.Results：The calibration curve of Icariin was linear in the range of 0.24～3.60μg（r=0.9997）,the averge of recovery was 98.30%,RSD=0.64%.Conclusions：The methods is simple and accurate,It can be used for quality control of Bushen Nuanyang granules.