大鼠离体心脏缺血预处理的核磁共振研究

^(31)P nuclear magnetic resonance surface coil study of ischemic preconditioned isolated perfused rat heart

缺血预处理 (IPC)是一种肌体对长时间缺血产生耐受性的处理技术。应用核磁共振表面线圈技术获得大鼠心肌在灌流的不同阶段的 31 P谱 ,可以从能量代谢的角度对缺血 /再灌注心脏进行连续、动态、无损伤的检测 ,从含磷代谢物的转化对缺血预处理产生作用的机理进行研究。实验结果显示 ,缺血预处理的保护作用并不是通过有利于心肌内 ATP和 PCr(磷酸肌酸 )恢复的水平来体现的 ,而是在缺血期通过缓和 p Hi(胞内 p H)值的突然变化和 ATP的大量消耗以及通过 p Hi 值和 ATP的尽快恢复来减轻心肌组织结构在缺血期产生的严重损伤 。

Ischemic preconditioning (IPC) will protect the heart from the damage caused by a subsequent long ischemia period. 31 P spectra of isolated perfused rat heart measured by the nuclear magnetic resonance (NMR) surface coil technique can be used to continually, dynamically and noninvasively obtain metabolism information. This paper explores the IPC mechanisms by NMR. This study shows that IPC has no effect on enhancing the ATP and PCr levels during reperfusion but makes significantly slows and smooths the changes of intracellular pH and ATP during ischemia periods. The ATP and PCr recovery rate of the IPC group after ischemia is significantly higher than that of the control group. In conclusion, the above results support that IPC can protect the rat heart by reducing damage during the ischemia period.