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大鼠切口痛模型中鞘内注射不同剂量KN93对瑞芬太尼诱发的痛觉过敏的影响 被引量：1

Effects of different dose of KN93 on remifentanil -induced hyperalgesia
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摘要 目的观察不同剂量KN93对瑞芬太尼痛觉过敏大鼠痛行为的影响。方法采用完全随机分组方法，将36只雄性SD大鼠分为6组（每组6只）：①空白对照组；②切口组；③瑞芬太尼组；④KN9325μg组；⑤KN9350μg组；⑥KN93100μg组。除空白对照组外，其余各组均制作切口痛模型、瑞芬太尼组及KN933个剂量组，手术过程中皮下输注瑞芬太尼（40μg/kg，1mg溶于40ml生理盐水中）30min。各组大鼠分别于术前24h、术后2、6、24、48h测定术侧机械缩足阈值（paw withdrawal mechanical threshold，PWMT）和热缩足潜伏期（paw withdrawal thermal latency，PWTL）。结果与切口组比较，瑞芬太尼组术后出现痛觉过敏表现PWMT（20．9±2．6，19．6±1．8，21．9±2．0，22．3±1．9）及肌[（8．5±1．5）s，（8．6±1．5）s，（8．5±2．0）s，（9．1±1．5）s]，P〈0．05。鞘内注射KN9325μg对痛觉过敏大鼠痛行为无改善作用，鞘内注射KN9350、100μg能剂量依赖性改善瑞芬太尼所致痛觉过敏大鼠痛行为，P〈0．05。结论瑞芬太尼诱发切口痛大鼠痛觉过敏；KN93剂量依赖性的改善瑞芬太尼诱发的痛觉过敏。 Objective To observe effects of different dose of （calcium/calmodulin-dependent protein kinase Ⅱ , CaMK Ⅱ） inhibitor KN93 on the hyperalgesia induced by remifentanil in rat model of incisional pain. Methods Thirty six Sprague Dawley rats were divided randomly into 6 groups with 6 rats each. Control; Incisional pain group; Remifentanil group; KN93 group （25 μg）; KN93 group （50 μg）; KN93 group （100 μg）. The model of incisional pain was performed in all groups except the control. In remifentanil group and KN93 （25, 50, 100 μg） groups, remifentanil （40 μg/kg） was given subcutaneously 30 min after the beginning of surgery. The paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） at the times of 24 h before and 2, 6, 24, 48 h after surgery were underwent. Results Compared with the incisional pain group ,the rats in the remifentanil group {the PWMT （20.86±2.55, 19.57±1.81, 21.87±2.01, 22.26±1.91） and the PWTL [ （8.47±1.52） s, （8.58±1.51） s, （8.45±2.05） s, （9.06±1.47） s ] showed sensory hyperalgesia （P〈0.05）. 25 μg KN93 had no effect on the hyperalgesia induced by remifentanil, while 50 μg and 100 μg KN93 can relieve the hyperalgesia induced by remifentanil （P〈0.05）. Conehmions Remifentanil can induce sensory hyperalgesia; Intrathecal injection KN93 may relieve the hyperalgesia dose-dependently.
作者 程崇学 赵鑫 张兢 顾小萍 马正良
机构地区 徐州医学院江苏省麻醉医学研究所&江苏省麻醉学重点实验室 南京大学医学院附属鼓楼医院麻醉科
出处 《国际麻醉学与复苏杂志》 CAS 2013年第4期320-323,共4页 International Journal of Anesthesiology and Resuscitation
基金 基金项目：国家自然科学基金（30872439/81171047） 六大人才高峰（2010117） 江苏省自然基金（BK2010105） 江苏省卫生厅课题（RE2011006/H200943） 南京市卫生局重大课题（zkx08028）
关键词 瑞芬太尼 切口痛 KN93 钙钙调素依赖性蛋白激酶Ⅱ Remifentanil Incisional pain KN93 Calcium/calmodulin-dependent protein kinase
分类号 R614 [医药卫生—麻醉学]
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参考文献15

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共引文献60

同被引文献12

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国际麻醉学与复苏杂志
2013年 第4期

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