二斑叶螨对阿维菌素敏感性的快速检测方法筛选

A rapid test to determine the sensitivity of Tetranychus urticae to abamectin

为了寻找一种快速检测二斑叶螨Tetranychus urticae Koch对阿维菌素敏感性的试验方法,本试验分别采用酶标板法、玻璃管法、离心管法、玻片浸渍法和培养皿封膜法,测定了阿维菌素处理后1、2、3和4 h时二斑叶螨的死亡率。结果发现,酶标板法和玻璃管法操作不方便;离心管法无法准确判断死亡与否;玻片浸渍法作用速度慢;培养皿封膜法方便快捷。据此得出,采用培养皿封膜法于处理后3 h观察结果,可以省时省力的测定二斑叶螨对阿维菌素的敏感性。

In order to find the best method for determining the sensitivity of Tetranychus urticae Koch to abamectin, the effectiveness of 5 assays; the microtiter plate method, the glass tube residual film method, the centrifugal tube residual film method, the slide-dip method and the cling-film covered petri dish method, was evaluated. The results show that the microtiter plate and glass tube residual film method were not convenient, and that the centrifugal tube residual film method barely determined whether T. urticae adult individuals were alive or dead. The slide-dip method was very slow but the cling-film covered petri dish method proved a convenient and efficient way to detect the sensitivity of T. urticae to abamectin 3 hours after treatment with this pesticide.