东亚飞蝗脑的形态学观察及三维重建

Morphological observation and 3D reconstruction of the brain of Locusta migratoria manilansis

本文主要研究东亚飞蝗Locusta migratoria manilansis(Meyen)脑部的形态结构及其三维重建模型。采用石蜡包埋切片,在光镜下观察了东亚飞蝗脑部的形态结构,其由前脑、中脑和后脑3部分组成。为了获得整只蝗虫的连续、完整的图像数据集,采用冰冻切片技术将冰冻包埋剂(OCT)包埋的飞蝗成虫做连续切片。然后利用图像处理方法对飞蝗脑部的连续切片进行配准、分割,再用三维重建软件Image-Pro Plus(IPP)对分割后的脑部二维图像序列进行三维重建,构建出的飞蝗脑部三维结构模型可以任意旋转,能从不同角度观察。其结果为蝗虫生理和防蝗治蝗提供科学依据。

The morphological structure of the brain of Locusta migratoria manilansis （Meyen） was observed and a 3D digital model of the brain developed. The brain was dissected and observed under light microscopy. The brain was found to consist of a protocerebrum, deutocerebrum and tritocerebrum. In order to obtain a 2D sectional image dataset of the locust, male adults were embedded in frozen embedding medium （OCT） and sliced into sections, and a 2D image of each section was stored in a dataset. After registering and segmenting the brain regions in the serial section images using image processing methods, Image-Pro Plus （IPP） was used to reconstruct a 3D model of the brain which can be freely rotated and observed from any angle. This model provides a basis for scientific research on grasshopper physiology and locust control.