基于准静态平面流场的新型皮肤组织工程灌注式生物反应室设计与初步模拟

Design and preliminary simulation of a new perfusion bioreactor for tissue-engineered skin based on quasi-static plane flow field

目的设计准静态平面流场的灌注式新型生物反应器,以适合组织工程皮肤的培养要求。方法采用计算流体动力学(computational fluid dynamics,CFD)商业软件FLUENT模拟新型生物反应器中的流场及培养支架壁面上的切应力,设计出一种能够层叠、低剪切力、均匀平面流场的生物反应器。结果模拟结果表明筛网状支撑物及组织工程皮肤构建物表面处的最大切应力小于3×10-5N/cm2,且培养室中的流场是均匀的。结论新型生物反应器满足准静态平面流场的要求,有可能在未来的皮肤组织工程研究和生产中广泛使用。

Objective To design and prepare a novel bioreactor based on the idea of the quasi-static plane flow field for perfusion culture in order to meet the requirement for culturing engineered-skin structures （ESS）. Methods A commercial software for computational fluid dynamics （CFD） analysis, FLUENT was used to mimic flow field and wall shear stress （WSS） in the new bioreactor. Based on this, a bioreactor with advantages of stack-up, low stress and homogeneous flat flow field was designed. Results The simulation results showed that the maximum WSS on mesh-like support and on the surface of tissue-engineered skin in the growth chamber was less than 3 x 10 -5 N/cm2＇ and the flow field in the bioreactor was uniform. The new biore- actor remedied some disadvantages in other bioreactors, such as the heterogeneous characteristics of flow, and the excess volume, etc. Our bioreactor met the requirement of gas-liquid interface and low stress for preparation of tissue-engineered skin. Conclusion Our new bioreactor satisfies the demands of quasi-static plane flow, and it may be suitable for culturing and producing the tissue-engineered skin in the future.