D2-40标记阳性淋巴管密度在非小细胞肺癌中的临床作用

Clinical application of D2-40-labeled positive expression of lymphatic vessel density in non-small cell lung cancer

目的探讨D2-40标记阳性淋巴管密度(LVD)在非小细胞肺癌(NSCLC)组织中的表达以及与临床病理特征的关系。方法采用免疫组化法检测46例NSCLC组织标本及10例肺良性病变组织标本中D2-40表达,计数D2-40阳性LVD,分析NSCLC周边区LVD与临床病理特征的关系。结果 NSCLC组织中肿瘤周边区LVD明显高于肿瘤中心区和良性病变组织[(11.83±4.15)个vs.(5.34±2.26)个和(4.73±2.62)个](P<0.05)。肿瘤周边区LVD与NSCLC的肿瘤大小、TNM分期及淋巴结转移相关(P<0.01),与患者的性别、年龄、病理类型以及肿瘤分化程度无关(P>0.05);肿瘤中心区LVD与上述临床病理参数均无明显相关性(P>0.05)。结论 D2-40可以特异性识别NSCLC组织中淋巴管内皮细胞;其标记的淋巴管主要位于癌周组织。LVD与NSCLC的肿瘤大小、临床分期及淋巴结转移密切相关,可用来评估肿瘤淋巴转移风险。

Objective To investigate the expression of D2-40-1abeled positive expression of lymphatic vessel density（LVD） in non-small cell lung cancer（NSCLC） and the correlation betweenLVD and clinicopathological characteristics. Methods The expression of D2-40 was detected by immunohistochemistry in 46 NSCLC cases and 10 cases with pulmonary benign lesions. The LVDpositively stained by D2-40 was counted, and the correlation between LVD in NSCLC borderline and elinicopatbologieal characteristic was analyzed. Results The LVD in tumor borderline was higher thanthat in tumor center tissues and benign lesions,respectively[（11. 83±4. 15） pieces vs. （5.34±2. 26） pieces and （4.73±2.62） pieces] （P〈0. 05）. The LVD in tumor borderline was positively correlatedwith tumor size, TNM stage and lymph node metastasis（P〈0. 01）, but was not significantly related to age, sex, histological type and differentiation degree （ P〉0. 05 ）. Moreover, there was no significantcorrelation between LVD in tumor center tissues and any clinicopathological factors. Conclusion D2-40 can specifically recognize the lymphatic endothelial cells in NSCLC tissues. The lymphaticvessel labeled by D2-40 mainly distributes in tumor borderline. LVD is closely correlated with tumor size,clinical stage and lymph node metastasis and may be taken as a risk factor for lymph nodemetastasis of NSCLC.