渗透树脂对乳、恒磨牙邻面早期龋损渗透能力的对比研究

Comparative study on the penetration abilities of resin infiltration into proximal initial caries lesions in primary molars and permanent posterior teeth

目的比较渗透树脂在乳、恒磨牙邻面早期龋损中的渗透能力。方法选取邻面呈白垩色改变的早期龋损离体乳磨牙32颗、恒牙(磨牙或前磨)36颗,垂直于龋损表面将牙齿切开,在体视显微镜下进行病损的组织学分级(C1～C4),C1:龋损位于釉质外1/2;C2:龋损位于釉质内1/2;C3:龋损位于牙本质外1/2;C4:龋损延伸至牙本质内1/2。C4被排除。随后病损用15%的盐酸酸蚀2 min后,进而使用渗透树脂渗透5 min。制作牙切片,使用双荧光染色技术,在激光扫描共聚焦显微镜(CLSM)下观察样本。分别测量乳、恒牙的病损深度(LD)和渗透树脂的渗透深度(PD),计算渗透百分数(PP),并对其进行统计学分析。结果在所有组织学病损分级(C1～C3级)中,乳、恒磨牙PP间的差异无统计学意义(P>0.05);但是,在C1组织学病损分级中,乳牙的渗透能力优于恒牙(P<0.05),在C2和C3组织学病损分级中,乳、恒牙PP间的差异无统计学意义(P>0.05)。结论渗透树脂对乳、恒磨牙邻面早期龋损均有很好的渗透作用。尤其对较浅的早期龋(C1级),渗透树脂治疗乳牙的效果优于恒牙。

Objective To compare the penetration abilities of resin infiltration into proximal lesions in primary molars and permanent posterior teeth in vitro. Methods Extracted or exfoliated primary molars （n=32） and perma- nent posterior teeth （n=36） with proximal white spot lesions were selected. Roots of the teeth were removed, and the crowns were cut across the caries lesions perpendicular to the surface. Cut surfaces were examined with stereo microscopy and classified with respect to histological lesion extension（C1-C4）： Lesions confined to the outer half on enamel （C1）, lesions confined to the inner half on enamel （C2）, lesions confined to the outer half on dentin （C3）, lesions extending into the inner half of dentin （C4）. C4 were excluded. Caries lesions were etched for 2 rain with 15% hydrochloric acid gel and were subsequently treated for 5 min with resin infiltration. Thin sections from each lesion were prepared and specimens were observed with confocal laser scanning microscopy （CLSM） through a dualfluorescence staining technique. Lesion depth （LD） and penetration depth （PD） of resin infiltration were measured in primary molars and permanent posterior teeth in confocal microscopic images, and percentage penetration （PP） were calculated respectively. The date were collected and analyzed. Results There were no significant differences for overall PP in primary molars and permanent posterior teeth groups （,PM）.05）. However, the penetration abilities in primary molars were slight higher than those in permanent posterior teeth in C1 group（P〈0.05）. The penetration abilities had no significant difference in primary molars and permanent posterior teeth in both C2 and C3 groups （PM）.05）. Conclusion Resin infiltration have satisfied penetration abilities in primary molars and permanent posterior teeth with proximalinitial lesions. The better penetration abilities of resin infiltration in primary molars are shown in C1 lesions than those in permanent posterior teeth.