6-羟基烟碱与烟碱的安全性比较研究

Study on the safety of 6-hydroxynicotine compared with nicotine

通过毒理学试验比较6-羟基烟碱与烟碱的安全性:对SPF级雄性小白鼠(体重30～35 g)进行急性毒性试验,分别进行35 d连续灌胃每只1.00 mg,0.75 mg,0.50 mg,0.25 mg的烟碱和6-羟基烟碱试验(分别相当于每d抽吸20支烟的烟民烟碱接受量的8倍,6倍,4倍,2倍);计算各组心肝脾肺肾的脏器系数,检测血液中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)3项生化指标,并对小鼠活体解剖取肝、肺、肾组织作病理学检查.结果表明,6-羟基烟碱相比烟碱的毒性明显降低(P<0.05),低剂量的烟碱不会造成小鼠器官的病变.

The safety of 6-hydroxynicotine and nicotine was compared through toxicology test. The acute tox- icity and accumulative test was carried out on SPF mice （each of 30 ~ 35 g）. After 35 d intragastrically each of 1.00 mg,0.75 mg,0.5 mg,0.25 mg of nicotine and 6-hydroxynicotine（ about receive the amount of a man pumping 20 cigarettes a day 8,6,4,2 times）, the organ coefficient of heart, liver, spleen, lung and kidney were calculated and bloodindicators of ALT, AST, TG were determined, the pathological examination was also done using liver, lung and kidney tissue. The results showed that 6-hydroxynicotine compared to nicotine significantly reduced in toxicity （ P 〈 0.05 ）, low doses of nicotine does not cause organ lesions in mice.