黄芪感染根腐病菌后抗氧化系统的变化规律研究

Studies on antioxidant system of Astragalus membranaceus after infected with Fusarium oxysporum

为了研究黄芪根腐病发病过程中的生理生化变化,在实验条件下人工接种尖孢镰刀菌使黄芪感病,每隔7d取样,连续测定了以下5个指标:超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性蛋白和丙二醛(MDA).结果表明,接种尖孢镰刀菌后,黄芪叶中SOD、CAT和POD活性均呈现出先升高后降低的变化规律,SOD和CAT活性在接菌后第7d达到峰值后逐渐下降,POD活性接菌后升高在第14d时达到峰值后活性下降;可溶性蛋白含量在叶中总体呈现出先升高后降低的趋势,在第14d时达到峰值;丙二醛(MDA)含量在叶中随接种时间的延长先逐渐增加,在第14d达到峰值后降低,且持续高于对照.

In order to study the physiological and biochemical changes of Astragalus membranaceus in the process of root rot, Astragalus rnernbranaceus is induced by being inoculated with Fusariurn oxysporum artificially under the experimental conditions, and the changes of 5 indixes including peroxidase（POD）, catalase（CAT）, superoxide dismutase（SOD）, soluble protein and malondialdehyde（MDA） are measured every seven days during the process. The results show that when the healthy Astragalus membranaceus become rotten gradually, the activities of peroxidase（POD）, catalase（CAT） and superoxide dismutase （SOD） increased first but decrease afterwards, and the activities of SOD and CAT peak within 7 days while the activity of POD peak within 14 days; the content of malondialdehyde（MDA） peak within 14 days and gradually decrease when the time for treating is prolonged; and the tendency of the content of soluble protain is similar to that of the content of malondialdehyde（MDA）.