凝胶组成和结构对淀粉糖化酶固定化效果的影响

Effect of the Structure and Functional Group on the Immobilizing of the Amylase into the Hydrogel

以凝胶作为酶的固定化载体,淀粉糖化酶为模型酶,考查不同组成和结构的凝胶对淀粉糖化酶的固定化效果.测定结果表明,聚丙烯酰胺凝胶中引入环氧基团和微粒复合结构可提高酶的固定量并改善固定化酶重复使用中的稳定性.具有复合结构的聚丙烯酰胺凝胶比小分子交联剂共价交联的常规凝胶有利于提高淀粉糖化酶的固定量和重复使用活性.

The effect of structure and composition of hydrogel on enzyme immobilization was inspected by using glucoamylase as model enzyme.The results showed that micro-particle composite structure and epoxy groups introduced into polyacrylamide hydrogel were both benefit for the enzyme immobilization in that the amount of enzyme immobilized increased and the stability of immobilized enzyme in repeated use was improved.It was also found that more glucoamylase was immobilized for composite-structured polyacrylamide hydrogel than that for small molecular crosslinked conventional hydrogel.The activity of immobilized enzyme in repeated use is higher for omposite-structured polyacrylamide hydrogel than that for small molecular crosslinker crosslinked conventional hydrogel.