海风藤对β淀粉样蛋白寡聚体激活小胶质细胞的影响

The effect of Piper kadsura on Aβ oligomer-induced inflammatory factor secretion from microglia

目的研究海风藤提取物对β淀粉样蛋白(Aβ)寡聚体诱导小胶质细胞激活的影响。方法模拟体内生理条件诱导Aβ单体形成Aβ寡聚体,原代培养新生大鼠小胶质细胞,分别用Aβ25-35寡聚体、Aβ25-35寡聚体+海风藤提取物、Aβ25-35寡聚体+DMSO干预小胶质细胞,并设空白对照组,48h后测上清液中白细胞介素1β(IL-1β)和白细胞介素6(IL-6)水平,并比较各组间炎性因子差异。结果 Aβ寡聚体组上清液中IL-1β、IL-6水平明显高于空白对照组〔IL-1β:(67.06±1.79)pg/mL vs.(36.30±1.40)pg/mL;IL-6:(181.14±4.46)pg/mLvs.(110.90±4.62)pg/mL,均P<0.05〕;Aβ寡聚体组上清液中IL-1β、IL-6水平明显高于Aβ寡聚体+海风藤提取物组〔IL-1β:(63.24±2.00)pg/mL,IL-6:(170.34±3.47)pg/mL,P<0.05〕;Aβ寡聚体组上清液中IL-1β、IL-6水平和Aβ寡聚体+DMSO组〔IL-1β:(68.26±1.38)pg/mL,IL-6:(184.7±6.06)pg/mL〕比较无统计学差异。结论 Aβ寡聚体能激活小胶质细胞;海风藤提取物能明显抑制Aβ寡聚体诱导小胶质细胞的激活。

Objective from mieroglia induced by Aβ without Piper kadsura extract. the Aβ25-35 oligomers group, DMSO group. 48 hours later, inflammatory factors between （36.30±1.40） pg/mL, IL-6： [ （181.14±4.46） pg/mL] in To explore the effect of Piper kadsura extract on inflammatory factor secretion oligomer. Methods Primary microglia was activated with Aβ oligomer with or The cultured microglial cells were divided into 4 groups： the blank control group, the Aβ25-35 oligomers± Piper kadsura extract group and the Aβ25-35 oligomers IL-I and IL-6 in the supernatant were measured by ELISA kit. The difference of each group was compared. Results Compared with the control group [IL-10 （110.9±4.62） pg/mL], the level of IL-1β [ （67.06±1.79） pg/mL] and IL 6 the supernatant of Aβ oligomer group increased significantly （P〈0.05） ; the level of IL-1β and IL-6 in the supernatant of Aβ oligomer group was significantly higher than that in the Aβ oligomer ± Piper kadsura extract group [-IL-1] （63.24±2.00） pg/mL, IL-6： （170.34±3.47） pg/mL, P〈0.05]. There was no difference between the level of IL-1β and IL-6 in the supernatant of Aβ oligomer group and Aβ oligomer DMSO group [IL-1β （68.26±1.38） pg/mL, IL-6： （184.7±6.06） pg/mL]. Conclusions Aβ oligomer can activate microglia Piper kadsura extract can significantly inhibite the A oligomer induced microglial activation.