2008～2011年贵州省肠道病毒71型分子流行特征分析

Analysis on Molecular Epidemiological Features of Enterovirus Type 71 in Guizhou Province,2008-2011

为研究贵州省肠道病毒71型(EV71)的基因型和分子流行特征,监测了全省报告的手足口病病例,选择2008年以来贵州全省部分EV71阳性标本进行病毒分离及VP1全基因测序(含重症病例、死亡病例和轻症病例),与国内外近年流行毒株及各亚型代表株进行基因比对,分析同源性及基因亚型。2008年、2009年及2011年贵州省流行的主要病原为EV71,获得109株参比序列毒株的同源性为95.3%～99.7%,贵州省毒株与邻省及山东省、上海市、南京市、吉林省和宁波市代表株的同源性最高,轻症与重死病例的核苷酸及氨基酸序列无明显的特征性差异,未出现不同基因亚型病毒的输入或改变,仍属C4a亚型。同地区、同年度内的核苷酸序列差异小于跨地区、跨年度差异。

To study the genotype and molecular epidemiological features of enterovirus type 71 （EV71） in Guizhou Province. The hand-foot-mouth disease（HFMD） patients since 2008 in Guizhou Province were surveyed. EV71 RNA positive samples, including mild cases, death cases and cases of serious conditions, were used for subsequent cell culture and sequencing of VP1 complete gene. Genotypes were compared between domestic and foreign epidemic strains. The results showed that the main pathogenwas EV71 subtype C4a in 2008, 2009 and 2011. The nucleotide homology was 95.3-99.7% among 109 sequenced strains isolated in Guizhou Province. Guizhou strains were more similar to the representative strains from neighbor Province or City, including Shandong, Shanghai, Nanjing, Jilin and Ningbo. There was no distinct sequence difference among the isolates from either of death, mild case or case of serious condition at the nucleotide and amino acid levels. The diversity of nucleotide sequence of isolates was less within same region and year than those trans-regional strains or strains in different year.