羊瘙痒因子263K感染仓鼠临床终末期脑中αB-晶体蛋白表达的研究

Analysis of The Expressions of αB-crystallin in The Brain Tissues of Agent 263K-infected Hamsters at Terminal Stage

αB-晶体蛋白(αB-crystallin)是小热休克蛋白(Small heat shock protein,sHSP)家族的代表成员,已发现与多种神经退行性疾病发生发展密切相关,但至今为止对αB-晶体蛋白在朊病毒病发生发展中可能扮演的角色研究甚少,作用尚不清楚。本研究利用羊瘙痒因子仓鼠敏感株263K感染仓鼠建立的朊病毒实验动物模型,通过免疫印迹(Western blots)和免疫组织化学染色观察到伴随PrPSc在感染终末期动物脑组织中大量沉积,αB-晶体蛋白表达显著上调,比正常对照增高3倍。免疫荧光双染确定其分布的主要细胞类型为星形胶质细胞,神经元中未检测到表达。感染动物中高表达的αB-晶体蛋白与异常沉积的PrPSc无明显共定位。此研究为进一步探讨和揭示αB-晶体蛋白在朊病毒病中可能的作用和分子机制奠定了基础。

αB-crystallin is a tant role in multiple neuro member of the sHSP （Small h degeneration diseases. To give of αB-crystallin in prion disease, the aB-crystallin levels eat shock protein） family, which plays an impor-insight into the possible alternation and the role in the brain tissues of agent 263K-infected hamsters were evaluated. Western blots and IHC revealed that at the terminal stages of the diseases, the levels of αB-crystallin were increased up to 3-fold in the brain tissues of scrapie infected 263K hamsters compared with normal controls. Immunofluorescent assays revealed that the up-regulated αB-crystallin was mainly observed in astrocytes, but not in neurons. The co-localization between ;B-crystallin and abnormal deposition of PrPsc in the brain tissues of the serapie infected hamsters was not observed. The study may provide a foundation for further revealing the potential role of αB-crystallin in prion disease.