摘要
呼吸道病毒感染是导致人群发病和死亡的主要原因之一。通过多重病原检测迅速筛查大量致病原,已成为呼吸道病毒感染检测的重要技术。本文介绍了呼吸道病毒多病原检测技术及一些对呼吸道病毒检测有潜在应用价值的新兴技术。这些技术包括多重呼吸道病毒快速分离培养技术、传统多重逆转录PCR技术、多重实时定量PCR技术、微芯片技术、质谱技术及宏基因组技术等。这些技术的发展和应用,不但有助于提高呼吸道病毒感染的快速诊断和治疗,而且也将促进新型呼吸道病毒的发现。
Viral respiratory tract infection is among the leading causes of mortality and morbidity world-wide. Rapid screening methods for multiple detection of a wider range of pathogens become very important for diagnosis of respiratory infection. This article describes conventional detection technologies and several emerging multiplex assays that have pot-ential applications in the diagnosis and monitoring of respiratory viral infections. These techniques include new rapid culture system, multiplex reverse transcription-PCR, real-time reverse transcription PCR, solid and suspension microarrays, mass spectrometry as well as metagenomics methods. The development and application of these techniques will not only improve the ability of rapid detection and control of viral respiratory infection, but play pivotal roles in the rapid charac-terization of new viral pathogens.
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第2期238-244,共7页
Chinese Journal of Virology
基金
CNIC-US CDC合作项目资助
项目名称:"Developing Sustainable Influenza Surveillance Networks and Response to Avian and Pandemic Influenza in China"
关键词
呼吸道病毒感染
多病原检测
检测方法
研究进展
Viral respiratory tract infection
Detection of multiple pathogens
Detection methods
Research advance