九种植物提取物对鼠永生化黑素细胞抗氧化能力的作用

Antioxidant Capacity of Nine Kinds of Plant Extracts on Mouse Immortalized Melanocyte

目的检测枸杞多糖、银杏叶提取物、山茶花提取物、蒺藜提取物、天麻提取物、槲皮素、白藜芦醇、茶多酚、人参皂苷rg2对鼠永生化黑素细胞(B10BR)抗过氧化氢(H2O2)诱导的氧化应激保护作用,并且检测其对酪氨酸酶活性的影响。方法建立过氧化氢损伤模型,分别设空白对照组、H2O2组、药物预处理+H2O2组。MTT法测细胞活性,流式细胞仪检测细胞内活性氧(ROS)水平,多巴氧化法测酪氨酸酶活性。结果 H2O2损伤模型中,半致死H2O2浓度是250μmol/L,筛选最佳作用浓度枸杞多糖为8 g/L、银杏叶提取物20μmol/L、山茶花提取物200 mg/L、蒺藜提取物0.2 g/L、天麻提取物0.8 g/L、槲皮素40μmol/L、白藜芦醇0.3μmol/L、茶多酚200 mg/L及人参皂苷50μmol/L,此9种植物提取物对H2O2诱导的黑素细胞损伤都有保护作用,且其降低ROS含量(P<0.01),增强细胞活性(P<0.01)。此外,枸杞多糖、蒺藜提取物、槲皮素、天麻提取物可抑制H2O2造成的酪氨酸酶活性降低(P<0.01)。结论此9种植物提取物均可保护黑素细胞,提高细胞抗氧化活性,而且有些药物能增强酪氨酸酶活性,这可能是其治疗白癜风的部分作用机制。

Objectlve To determine the wolfberry polysaccharide, ginkgo biloba extract, camellia extract, Tribulus Terrestris extract, Tianma extract, quercetin, resveratrol, polyphenols, ginsenosides on mouse immortalized melanocytes （B10BR） against H202-induced oxidative stress protective effect, and to detect its impact on the activity of tyrosinase. Methods Hydrogen peroxide injury model was established and divided into blank control group, H202 group, pharmacological preconditioning ＋ H202 group. MTF method was used to measure cell activity, flow cytometry detection of intraeellular reactive oxygen species （ROS）, dopa oxidation method to measure the tyrosine activity. Results hydrogen peroxide injury model, the optimum concentration of H202 is 250 μmol/L, selecting the optimal concentration woliberry polysaccharide 8 g/L, ginkgo biloba extract 20 μmol/L, camellia extract 200 mg/L, Tribulus terrestris extract 0.2 g/L Fraction 0.8 g/L, quercetin 40 μmol/L the resveratrol 0.3 μmol/L polyphenols 200 mg/L and ginseng the saponin 50 μmol/L this nine kinds of natural medicines on the hydrogen peroxide-induced melanocyte damage had a protective effect, and lower levels of ROS（P〈0.01 ） and enhanced cell activity（P〈0.01 ）. In addition, LBP, Tribulus Terrestris extract, quercetin, Tianma extract can inhibit tyrosinase activity reduces caused by H202 （P〈0.01）. Conclusion The nine natural medicines can inhibit apoptosis of melanocytes to enhance cell antioxidant activity, and some drugs could enhance the activity of tyrosinase, which might be part of the mechanism of action for the treatment of vitiligo.