siRNA干扰CLEC2B基因沉默对黑素细胞的影响

Effects of RNA Interference Inducing CLEC2B Gene Silence on Melanocytes

目的探讨小干扰RNA(small interfering RNA,siRNA)诱导淋巴细胞中CLEC2B(C-type lectin domain family 2,member B)基因沉默后,其淋巴细胞上清液对黑素细胞增殖活性的影响,从而揭示CLEC2B基因参与白癜风发病的免疫学机制。方法选用Jurkat淋巴瘤细胞及B16黑素瘤细胞为研究对象,应用RNA干扰技术沉默淋巴细胞CLEC2B mRNA的表达,MTT法检测CLEC2B基因沉默后淋巴细胞增殖情况及其上清液作用后对黑素细胞增殖情况。结果CLEC2B基因沉默后淋巴细胞增殖无明显影响,CLEC2B基因沉默后淋巴细胞上清液对黑素细胞的抑制作用降低,黑素细胞存活率增加(P<0.05)。结论 CLEC2B基因表达下调后减弱对黑素细胞的抑制作用,提示CLEC2B基因影响黑素细胞增殖活性,从而参与白癜风发病。

Objective To observe effect on that whether lymphocytes＇ supernatant could influence melanocytes proliferation or not when CLEC2B gene expression is silenced by establishing the lymphocytes transient silencing system, representing CLEC2B participation path in the immunity mechanism of vitiligo. Methods The lymphocytes lines Jurkat and melanocytes lines B16 were selected. CLEC2B gene was silenced by RNA interference technique, the proliferation of melanocytes and lymphocytes were detected by MTF. Results The lymphocyte proliferation had no significantly change, lymphocytes＇ supernatant could decrease the suppression to melanocytes proliferation （P〈0.05）, so as to create beneficial environment for melanocytes living rate. Conclusion Silencing CLEC2B gene could influence melanocytes proliferation by the way of affecting lymphocytesI function. It shows that CLEC2B is participated in the immune-pathogenesis.