中国人群eNOS基因内含子13微卫星多态性的初步研究

Preliminary investigation of the microsatellite at the endothelial nitric oxide synthase gene intron 13 in Chinese

目的 :为研究eNOS基因内含子 13上的微卫星序列与妊高症的相关性。方法 :采用PCR-PAGE与银染相结合的方法 ,研究其多态性。结果 :得到含有该微卫星序列的DNA片段。结论 :证实在中国人群中eNOS基因内含子 13上的确存在一个CA重复的多态性微卫星序列 ,从而为研究该位点与妊高症的相关性提供了技术支持和理论依据。

Aim:To investigae the relation between the microsatellite of eNOS gene intron 13 and pregnancy-induced hypertension (PIH), the polymorphism of this microsatellite is first studied. Methods: the polymorphism is studied by PCR-PAGE and silver-stained method. Results: the fragment including microsatellite sequence is obtained. Conclusion: the results showed that there is a microsatellite-polymorphism at eNOS gene intron 13 in Chinese, this would provide support and theoretical base for further analysis of the relation between this polymorphism and PIH. [