不同补肾法在脑梗死大鼠体内促骨髓间充质干细胞向神经元样细胞分化的作用

Effect of different kidney-nourishing method on the differentiation of bone marrow mesenchymal stem cells to neuron-like cells in cerebral infarction rats

目的探讨不同补肾法在脑梗死大鼠体内对骨髓间充质干细胞(BMSCs)向神经元样细胞分化的影响。方法用线栓法制备大鼠大脑中动脉梗塞(MCAO)模型,随机分为空白诱导组、左归丸诱导组、地黄饮子诱导组、右归丸诱导组、维甲酸(RA)诱导组、左归丸联合RA诱导组、右归丸联合RA诱导组、地黄饮子联合RA诱导组,不同补肾法代表方药含药血清孵育BMSCs,移植后7、14 d进行移植细胞神经标志蛋白的检测。结果诱导BMSCs经静脉移植入大鼠体内后,阳性表达神经细胞的相关蛋白神经元特异性烯醇化酶(NSE)、Nestin及胶质纤维酸性蛋白(GFAP)。结论不同补肾法在脑梗死大鼠体内能够促BMSCs向神经元样细胞分化,且补阴类代表方左归丸和阴阳双补类代表方地黄饮子在体内诱导分化的效果优于补阳类代表方右归丸。

Objective To discuss the effect of different kidney-nourishing method on the differentiation of bone marrow mesenchymal stem cells （BMSCs） in cerebral infarction rats to sample neurons cell. Methods Suture ligation method was used to prepare middle cere-bral artery obstruction （MCAO） model, which were randomly divided into blank induction, zuoguiwan induction, dihuangyinzi induction, youguiwan induction, retinoic acid induction, zuoguiwan and retinoic acid induction, youguiwan and retinoic acid induction, dihuangyinzi and retinoic acid induction groups. Medicine-containing serum of different kidney-nourishing prescription was used to incubate BMSCs. In the 7th and lgth day after transplantation, transplant cellular neural mark protein detection was conducted. Results After induced BMSCs were intravenously transplanted into the rats, positive expression of nerve cell-related proteins NSE, Nestin and GFAP were found. Conclu- sions Different kidney-nourishing methods can promote BMSCs differentiation in the cerebral obstruction rats to neuron-like cells, and yin-nourishing representative zuoguiwan and yinyang-conourishing representative dihuangyinzi have better effects of induced differentiation than that of yang-nourishing representative youguiwan.