白藜芦醇干预对已退变椎间盘终板软骨细胞SIRT1表达的影响

Duration-and concentration-dependent induction of SIRT1 on human degenerative endplate chondrocytes by Resveratrol

目的探讨不同浓度及不同时间的白藜芦醇干预对退变椎间盘终板软骨细胞沉默信息调节因子2相关酶1(SIRT1)表达的影响。方法老年患者的终板软骨细胞培养、传代,P2代细胞随机分组,分别用不同浓度及不同作用时间的白藜芦醇干预,终止培养后检测SIRT1蛋白及mRNA表达水平。结果与空白对照组相比较,DMSO组SIRT1蛋白及mRNA表达无显著差异(P>0.05);白藜芦醇干预的浓度在12.5μmol/L、作用24 h,以及浓度在50μmol/L、作用12 h以上时能够显著促进已退变椎间盘终板软骨细胞表达SIRT1蛋白及mRNA(P<0.05)。结论白藜芦醇干预可促进退变椎间盘终板软骨细胞表达SIRT1蛋白及mRNA,且该作用呈现浓度、时间依赖关系;0.1%DMSO对细胞表达SIRT1蛋白无抑制作用。

Objective To explore the effect of different concentrations and time of Resveratrol intervention on the expression of SIRT1 in human degenerative endplate chondrocytes. Methods Degenerative disc endplate chondrocytes （DEC） were harvested from eider patient with lumbar disc hernia, and then cultured. Passage 2 DEC were stimulated by Resveratrol of different concentrations and duration re-spectively, and then expression levels of SIRT1 protein and mRNA were detected by the end of stimulation. Results Compared with the ex-pression levels of SIRT1 and of SIRT1 mRNA in control group, there was no significant difference in DMSO group （P 〉 O. 05）. Resveratrol of 12. 5 μmol/L and 24 hours, Resveratrol of 50 μmol/L and 12 hours could stimulate the expressions of SIRT1 protein and mRNA （ P 〈 0. 05）. Conclusions Resveratrol intervention can significantly stimulate the expressions of SIRTI protein and mRNA in human DEC in a concentration-and time-dependent manner. 0. 1% DMSO has no inhibiting effect of SIRT1 protein expression.