MMP、TIMP在颅内动脉瘤中表达的实验研究

Matrix metalloproteinases and tissue inhibitors of metalloproteinases expressions in human intracranial aneurysm

目的本研究探讨基质金属蛋白酶（MMP）及基质金属蛋白酶抑制剂在颅内动脉瘤中的基因表达及作用。方法将30例颅内动脉瘤患者分为破裂动脉瘤组及未破裂动脉瘤组，均开颅行夹闭术，术后获得动脉瘤标本，real time RT—PCR法定量测定MMPs、TIMPs mRNA水平。ELISA法测定破裂动脉瘤与未破裂动脉瘤患者血清中MMP-2、MMP-9水平。免疫组织化学染色定位MMP、TIMP在组织细胞的位置。结果与未破裂动脉瘤相比，MMP-2、MMP-9在破裂动脉瘤中明显升高（P〈0．05），MMP-2／TIMP—1，MMP-2／TIMP-2，MMP-2／TIMP-3，MMP-9／TIMP-2在破裂动脉瘤中明显升高（P〈0．05）。破裂动脉瘤患者血清MMP-2、MMP-9水平明显高于未破裂动脉瘤（P〈0．05）。结论破裂和未破裂动脉瘤之间MMP表达水平差异与动脉瘤破裂相关。

Objective To explore the role of matrix metalloproteinase and tissue inhibitors of metalloproteinases in cerebral aneurysm. Metbeods Tissue samples of the cerebral aneurysms were obtained from 30 patients who underwent cerebral aneurysm clipping operation. We use real - time RT - PCR method to quantatively measure mRNA levels of MMPs and TIMPs. ELISA method was used to measure the serum levels of MMP - 2, MMP - 9 in cerebral ruptured aneurysm patients and unruptured aneurysm. The localization of MMPS and TIMPS in tissue and cellular was examined by immunohistochemical staining. Results Comparing with unruptured aneurysm, MMP-2 and MMP-9 were overexpressed in ruptured aneurysm. The ratos of MMP - 2/TIMP - 1, MMP - 2/TIMP - 2, MMP - 2/TIMP - 3, MMP - 9/TIMP - 2 were higher in cerebral ruptured aneurysms than in unruptured aneurysms. Patients with cerebral ruptured aneurysm had higher serum MMP-2, MMP-9 levels than those unruptured aneurysm. Conclusion The difference of MMP expression level between ruptured and unruptured aneurysm is related with aneurysm rupture.