摘要
目的探讨小鼠胚胎神经管畸形(NTDs)中过量维甲酸(RA)致畸及牛磺酸干预的作用,分析Pax3与NTDs的关系及其特异性时相表达。方法采用孕鼠服用过量RA及牛磺酸,建立胎鼠NTDs及干预动物模型;用免疫组化及逆转录聚合酶链式反应方法,分析各组胎鼠E9.5、E10.5、E11.5、E12.5中神经管Pax3的表达特异时相性差异。结果致畸组胎鼠畸形率达到71.6%,而干预组畸形率降至55.3%(P〈0.05),两组死胎或吸收胎率分别为11.4%、9.8%(P〉0.05);Pax3蛋白质阳性反应主要位于胞核,其在神经嵴表达强,而周围间充质表达弱。且在对照组中表达强于两实验组(P〈0.05),于E10.5d出现表达高峰;在两实验组中Pax3表达呈现增强趋势,干预组Pax3表达强于致畸组(P〈0.05);Pax3基因在两实验组中的表达受抑,与对照组比较明显减弱(P〈0.05),对照组中Pax3基因的表达于E10.5d出现高峰;在两实验组中Pax3随胚胎发育呈现增强趋势无表达高峰,干预组Pax3表达强于致畸组(P〈0.05)。结论RA致畸及牛磺酸干预在胚胎NTDs发生发展中的作用,与Pax3基因表达的时相差异性有关。
Objective To investigate the excess retinoic acid (RA) teratogenesis and taurine intervention in the embryonic neural tube defects (NTDs), and analyze the Pax3 specific phase expression in NTDs. Methods Feed excess retinoic acid and tanrine to pregnancy mice and set up the models of the fetal neural tube defects and intervention mice. Use the methods of immunohistochemistry and reverse transcription - polymerase chain reactor to analyze the Pax3 specific phase expression in each group of E9. 5, El0.5, Ell. 5, E12.5 fetal rats during neural tube development. Results The malformation rate in teratogenic group is 71.6%, while in intervention group it drops to 55.3% ( P 〈 0. 05 ). The rates of the stillbirth and absorption of the two groups were 11.4% and 9. 8% ( P 〉 0. 05 );the Pax3 protein positive immunostaining mainly located on the nucleus; the expression of Pax3 protein in the neural crest is stronger than those in the mesenchyme. Pax3 protein expression in the control group is stronger than those in the two experimental groups ( P 〈 0.05 ), and the peak expression appeared at E10. 5. Among the two experimental groups, Pax3 expression showed increasing trend , and its expression in the intervention group is stronger than the teratogenic group ( P 〈 0. 05 ). The Pax3 gene expression is inhibited in the two experimental groups, is significantly decreased comparing with the control group ( P 〈 0. 05 ) , and the peak expression of Pax3 gene appeared at E10. 5 in the control group; Among the two experimental groups, Pax3 expression showed increasing trend with the embryos development, and in the intervention group, it is stronger than the teratogenie group ( P 〈 0. 05 ) . Conclution The role of retinoic acid teratogenie and taurine intervention on the NTDs development was associated with the Pax3 gene specific phase expression.
出处
《中华神经外科杂志》
CSCD
北大核心
2013年第3期303-307,共5页
Chinese Journal of Neurosurgery
基金
宁夏自然科学基金(NZ0142)